Muroran Marine Station FSC Hokkaido University


In our laboratory, we study about the cell biology and molecular biology of brown algae. Brown algae classified to Stramenopile possess complicated life cycle and reproductive mechanisms. These marine organisms are responsible for the carbon fixation in costal marine environment. Furthermore, they are capable of absorbing heavy metals, Nitrogen and Phosphorus and it might be possible to use them for bioremediation.
Brown algae are known as one of the biggest aquatic photosynthetic organisms and their unique cell walls composed of polysaccharide enable to support their huge body. We are now focusing on 6 topics to understand the system of formation and maintenance for the multicellularity in brown algae; 1) formation of cytokinesis plane, 2) function and structure of the plasmodesmata, 3) proteomics of cell wall proteins, 4) structures and formation mechanisms of reproductive cells, 5) recognition of male and female gametes and 6) multiple culture conditions for effective maturation and growth.

proteomics of cell wall proteins

Under construction.

Proteomics of cell wall proteins

Structures and formation mechanisms of reproductive cells

Proteomics of cell wall proteins

Multiple culture conditions for effective maturation and growth

Recognition of male and female gametes

Multiple culture conditions for effective maturation and growth




3.Mazéas, L., Yonamine, R., Barbeyron, T., Henrissat, B., Drula, E., Terrapon, N., Nagasato, C., & Hervé, C. (2022, March). Assembly and synthesis of the extracellular matrix in brown algae. In Seminars in Cell & Developmental Biology. Academic Press.
2.Shen, Y., Motomura, T., & Nagasato, C. Ultrastructural observations of mitochondrial morphology through the life cycle of the brown alga, Mutimo cylindricus (Cutleriaceae, Tilopteridales) Protoplasma 259, 371-383.
1.Kim, G. H., Nagasato, C., Kwak, M., Lee, J. W., Hong, C. Y., Klochkova, T. A., & Motomura, T. Intercellular transport across pit-connections in the filamentous red alga Griffithsia monilis. Algae 37, 75-84.


5.Ichihara,K., Yamazaki, T. and Kawano, S. Genome editing using a DNA-free clustered regularly interspaced short palindromic repeats-Cas9 system in green seaweed Ulva prolifera. Phycological Researchm 70, 50-56. 10.1111/pre.12472
4.Badis, Y., Scornet, D., Harada, M., Caillard, C., Godfroy, O., Raphalen, M., Gascon, C., Coelho, S., Motomura, T., Nagasato, C., & Cock, J. M. Targeted CRISPR-Cas9-based gene knockouts in the model brown alga Ectocarpus. New Phytologist 231, 2077-20913. 10.1111/nph.17525
3.Yonamine, R., Ichihara, K., Tsuyuzaki, S., Hervé, C., Motomura, T., & Nagasato, C. Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes. Journal of Phycology 57, 1356-1367.
2.Zhang, J., Yotsukura, N., Jueterbock, A. Hu, Z., Assis, J., Nagasato, C., Yao, J. & Duan, D. Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina japonica. J Appl Phycol 33, 693-702 .
1.Shen, Y., Iwao, T., Motomura, T. & Nagasato, C. Cytoplasmic inheritance of mitochondria and chloroplasts in the anisogamous brown alga Mutimo cylindricus (Phaeophyceae). Protoplasma 258, 19-32.


2.Choi, J.W., Graf, L., Peters, A.F., Cock, J.M., Nishitsuji, K., Arimoto, A.,Shoguchi, E., Nagasato, C., Choi, C.G. & Yoon, H.S. Organelle inheritance and genome architecture variation in isogamous brown algae. Sci Rep 10, 2048.
1.Nagasato, C., Kawamoto, H., Tomioka, T., Tsuyuzaki, S., Kosugi, C., Kato., T & Motomura, T. Quantification of laminarialean zoospores in seawater byreal-time PCR. Phycological Research 68:57–62.


7.Miyamura, S., Ichihara, K., Yamazaki, T., Kuwano, K. & Kawano, S. Visualization of Gamete Mating Structure of Marine Green Alga by FE-SEM. Cytologia 9: 13523.
6.Mignerot, L., Nagasato, C., Peters, A., Perrineau, M., M., Scornet, D., Pontheaux, F., Djema, W., Roze, D., Motomura. T., Coelho, S., M., Cock, J., M. Unusual patterns of mitochondrial inheritance in the brown alga Ectocarpus. Molecular Biology and Evolution 36: 2778–2789.
5.Ichihara, K., Yamazaki, T., Miyamura, S., Hiraoka, M. & Kawano, S. Asexual thalli originated from sporophytic thalli via apomeiosis in the green seaweed Ulva. Scientific Report 9: 13523.
4.Zhang, J., Yao, J., Hu, Z.‐M., Jueterbock, A., Yotsukura, N., Krupnova, T., N., Nagasato., C. & Duan, D. Phylogeographic diversification and postglacial range dynamics shed light on the conservation of the kelp Saccharina japonica. Evolutionary Applications. 12:791–803.
3.Sahul, H., S., Wakayama, M., Ichihara, K., Sakurai, K., Ashino, Y., Kadowaki, R., Soga, T. and Tomita, M. Metabolome profling of various seaweed species discriminates between brown, red, and green algae. Planta 249: 1921-1947
2.Kinoshita-Terauchi, N., Shiba, K., Umezawa, T., Matsuda, F., Motomura, T. and Inaba, K. A brown algal sex pheromone reverses the sign of phototaxis by cAMP/Ca2+-dependent signaling in the male gametes of Mutimo cylindricus (Cutleriaceae). Journal of Photochemistry and Photobiology B: Biology 192: 113-123
1.Klochkova, T., A., Motomura, T., Nagasato, C., Klimova, A., V. and Kim, G., H. The role of egg flagella in the settlement and development of zygotes in two Saccharina species. Phycologia. Published online.


4.Zhang, J., Yao, J. Hu, Zi-Min, Jueterbock, A., Yotsukura, N., Krupnova, T. Nagasato, C. and Duan, De-Lin. (2018) Phylogeographic diversification and postglacial range dynamics shed light on the conservation of the kelp Saccharina japonica . Evolutionary Applications .12:791–803
3.Cui, J., Monotilla, A., P., Zhu, W., Takano, Y., Shimada, S., Ichihara, K., Matsui, T., He, P., and Hiraoka, M. (2018) Taxonomic reassessment of Ulva prolifera (Ulvophyceae, Chlorophyta) based on specimens from the type locality and Yellow Sea green tides. Phycologia 57: 692-704.
2.Nagasato, C. and Motomura, T. (2018) Effect of brefeldin A and the dynamics of the actin plate on cytokinesis of zygotes in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). European Journal of Phycology.
1.Nagasato, C., Katsaros, C. and Motomura, T. (2018) Cryofixation of brown algae for transmission electron microscopy. In Protocols for macroalgae research [eds Charrier, B., Wichard, T. and Reddy, C. R. K.], CRC Press. Taylor & Francis Group.


9.Godfroy, O., Uji, T., Nagasato, C., Lipinska, A. P., Scornet, D., Peters, A. F., Avia, K., Colin, S., and Mignerot L., Motomura, T., Cock, J. M. and Coelho, S. (2017) DISTAG/TBCCd1 is required for basal cell fate determination in Ectocarpus. Plant Cell. DOI 10.1105/tpc. 17.00440.
8.Yamazaki, T., Ichihara, K., Suzuki, R., Oshima, K., Miyamura, S., Kuwano, K., Toyoda, A., Suzuki, Y., Sugano, S., Hattori, M. & Kawano, S. (2017) Genomic structure and evolution of the mating-type locus in the green seaweed Ulva partita. Scientific Reports 71(1): 11679. doi: 10.1038/s41598-017-11677-0.
7.Hiraoka, M., Ichihara, K., Zhu, W., Shimada, S., Oka, N., Cui, J., Tsubaki, S. & He, P. (2017) Examination of species delimitation of ambiguous DNA-based Ulva (Ulvophyceae, Chlorophyta) clades by culturing and hybridization. Phycologia 56 (5): 517-532.
6.Masakiyo, Y., Ogura, A., Ichihara, K., Yura, K. & Shimada S. (2017) RNA-seq analysis revealed molecular mechanism for low-salinity adaptation between closely related ulvarian seawater and brackish water distributed species. Algal Resourses 9: 61-76.
5.Saber, A., A., Ichihara, K. & Cantonati, M. (2017) Molecular phylogeny and detailed morphological analysis of two freshwater Rhizoclonium strains from contrasting spring types in Egypt and Italy. Plant biosystems 151: 800-812.
4.Kinoshita, N., Nagasato, C. and Motomura, T. (2017) Phototaxis and chemotaxis of brown algal swarmers. Journal of Plant Research DOI 10.1007/s10265-017-0914-8.
3.Kinoshita, N., Nagasato, C. and Motomura, T. (2017) Calcium control of the sign of phototaxis in brown algal gametes of Mutimo cylindricus. Photochemistry and Photobiology (in press).
2.Iwata, I., Kimura, K., Tomaru, Y., Motomura. T., Koike, K., Koike, K. and Honda, D. (2017) Bothrosome formation in Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles) during zoospore settlement. Protist 168: 206-219.
1.Tanaka, A., Hoshino, Y., Nagasato, C. and Motomrua, T. (2017) Branch regeneration induced by sever damage in the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae). Protoplasma 254:1341-1351.



Kinoshita, N., Fu, G., Ito, T. and Motomura, T. (2016) Three-dimensional organization of flagellar basal apparatus in Ectocarpus gametes. Phycological Research 64:19-25.


Kinoshita, N., Nagasato, C., Tanaka, A. and Motomura, T. (2016) Chemotaxis in the anisogamous brown alga Mutimo cylindricus. Phycologia 55:359–364.


Yamada, K., Yoshikawa, S., Ohoki, K., Ichinomiya, M., Kuwata, A., Motomura, T., Nagasato, C. (2016) Ultrastructural analysis of siliceous cell wall regeneration in Triparma laevis (Parmales, Bolidophyceae). Phycologia. 55: 602-609


Yamada, K., Nagasato, C., Motomura, T., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K. and Yoshikawa, S. (2016) Mitotic spindle formation in Triparma laevis NIES-2565 (Parmales, Heterokontophyta). Protoplasma. DOI 10.1007/s00709-016-0967-x


Kinoshita, N., Nagasato, C. and Motomura, T. (2016). Chemotactic movement in sperm of the oogamous brown algae, Saccharina japonica and Fucus distichus. Protoplasma. DOI 10.1007/s00709-016-0974-y.


Terauchi, M., Nagasato, C., Inoue, A., Ito, T and Motomura, T. (2016). Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae). Planta DOI 10.1007/s00425-016-2516-4.



Tanaka A, De Martino A, Amato A, Montsant A, Mathieu B, Rostaing P, Tirichine L, Bowler C. (2015) Ultrastructure and Membrane Traffic During Cell Division in the Marine Pennate Diatom Phaeodactylum tricornutum. Protist 166:506-521.


Zhang, J., Yao, J. T., Sun, Z. M., Fu, G., Galanin, D. A., Nagasato, C., Motomura, T., Hu, Z. M. and Duan, D. L. (2015) Phylogeographic data revealed shallow genetic structure in the kelp Saccharina japonica (Laminariales, Phaeophyta). BMC Evol. Biol. DOI. 10.1186/s12862-015-0517-8.


Iwai, H., Fukushima, M., Yamamoto, M. and Taizo Motomura, T. (2015) Seawater extractable organic matter (SWEOM) derived from a compost sample and its effect on the serving bioavailable Fe to the brown alga, Saccahrina japonica. Humic Substances Research. Humic Sub. Res. (In press)


Fu G, Nagasato C, Yamagishi T, Kawai H, Okuda K, Takao Y, Horiguchi T and Motomura T. (2015). Ubiquitous distribution of helmchrome in phototactic swarmers of the Stramenopiles. Protoplasma (In press)


Kinoshita N, Shiba K, Inaba K, Fu G, Nagasato C and Motomura T. (2015). Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus. Eur. J. Phycol. DOI 10.1080/09670262.2015.1109144.


Bailleul B, Berne N, Murik O, Petroutsos D, Prihoda J, Tanaka A, Villanova V, Bligny R, Flori S, Falconet D, Krieger-Liskay A, Santabarbara S, Rappaport F, Joliot P, Tirichine L, Falkowski P, Cardol P, Bowler C, Finazzi G. (2015). Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms. Nature. 524:366-369


Klochkova T A, Shin Y J, Moon K H, Motomura T and Kim G H. (2015). New species of unicellular obligate parasite, Olpidiopsis pyropiae sp. nov., that plagues Pyropia sea farms in Korea. J. Appl. Phycol. DOI 10.1007/s10811-015-0595-4.


Nagasato C, Terauchi M, Tanaka A and Motomura T. (2015). Development and function of plasmodesmata in Fucus zygotes. Botanica Marina. 58: 229–238.


Villar E, Farrant G, Follows M, Garczarek L, Speich S, Audic S, Bittner L, Blanke B, Brum JR, Brune C, Casotti R, Chase A, Dolan JR, d'Ortenzio F, Gattuso JP, Grima N, Guidi L, Hill CN, Jahn O, Jamet JL, Le Goff H, Lepoivre C, Malviya S, Pelletier E, Romagnan JB, Roux S, Santini S, Scalco E, Schwenck SM, Tanaka A, Testor P, Vannier T, Vincent F, Zingone A, Dimier C, Picheral M, Searson S, Kandels-Lewis S, Tara Oceans coordinators, Acinas SG, Bork P, Boss E, de Vargas C, Gorsky G, Ogata H, Pesant S, Sullivan MB, Sunagawa S, Wincker P, Karsenti E, Bowler C, Not F, Hingamp P and Iudicone D. (2015). Environmental characteristics of Agulhas rings affect inter-ocean plankton transport. Science. 348: 6237


Huysman MJJ, Tanaka A, Bowler C, Vyverman W and De Veylder L. (2015). Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage. BMC Plant Biol. in press.


Terauchi, M, Nagasato, C, and Motomura, T. (2015). Plasmodesmata of brown algae. J. Plant Res. 128:7-15.

2. Idei, M, Sato, S, Nagasato, C, Motomura, T, Toyoda, K, Nagumo, T, amnd Mann, D, G. (2015). Spermatogenesis and auxospore structure in the multipolar centric diatom Hydrosera. J. Phycol. 51, 144-158.
1.Iwai, H., Fukushima, M., Motomura, T., Kato, T., Kosugi, C. (2014) Effect of iron complexes with seawater extractable organic matter on oogenesis in gametophytes of brown macroalgae (Saccharina japonica). J. Appl. Phycol. 27:1583–1591.


1.Iwai, H., Fukushima, M., Motomura, T., Kato, T., Kosugi, C. (2014) Effect of iron complexes with seawater extractable organic matter on oogenesis in gametophytes of brown macroalgae (Saccharina japonica). J. Appl. Phycol. 10.1007/s10811-014-0479-z.
2. J. Morrissey, R. Sutak, J. Paz-Yepes, A. Tanaka, A. Moustafa, A.j Veluchamy, Y. Thomas, H Botebol, F.Y. Bouget, J.B. McQuaid, L. Tirichine, A.E. Allen, E.l Lesuisse, and C.Bowler (2014) A Novel Protein, Ubiquitous in Marine Phytoplankton, Concentrates Iron at the Cell Surface and Facilitates Uptake. Curr. Biol. 25: 364-371.
3.Jong Won Han, J. W., Tatyana A. Klochkova, T. A, Shim, J., Nagasato, C. Motomura, T., and Kim, G. H. (2014) Identification of three proteins involved in fertilization and parthenogenetic development of a brown alga, Scytosiphon lomentaria. Planta. DOI 10.1007/s00425-014-2148-5.
4.Fu, G., Nagasato, C., Oka, S., Cock, M. J. and Motomura, T. (2014) Proteomics analysis of heterogeneous flagella in brown alga (Stramenopiles). Protist. DOI:10.1016/j.protis.2014.07.007.
5.Nagasato, C., Kajimura, N., Terauchi, M., Mineyuki, Y. and Motomura, T. (2014) Electron tomographic analysis of cytokinesis in the brown alga Silvetia babingtonii (Fucales, Phaeophyceae). Protoplasma DOI 10.1007/s00709-014-0635-y.
6.Sato, T., Nagasato, C., Hara, Y. and Motomura, T. (2014) Cell cycle and nucleomorph division in Pyrenomonas helgolandii (Cryptophyta).Protist 165:113-122.
7.Fu, G., Kinoshita, N., Nagasato, C. and Motomura, T. (2014) Fertilization of brown algae: Flagellar function in phototaxis and chemotaxis. In Sexual Reproduction in Animals and Plants. (eds. Sawada, H. Inoue, N., and Iwano, M.) Springer Open.


1.Klochkova, T., Kwak, M-S, Han, J-Won, Motomura, T., Nagasato, C. and Kim, G-H. (2013) Cold-tolerant strain of Haematococcus pluvialis (Haematococcaceae, Chlorophyta) from Blomstrandhalv?ya (Svalbard).
Algae 28, 185-192.


1.Kitauchi, T., Saito, T., Motomura, T., Arai, K., Yamaha, E. (2012) Distribution and function of germ plasm in cytoplasmic fragments from centrifuged eggs of the goldfish, Carassius auratus. J. Appl. Ichthyol.28:998-1005.
2.Terauchi, M., Nagasato, C., Kajimura, N., Mineyuki, Y., Okuda, K., Katsaros, C., Motomura. T. (2012) Ultrastructural Study of Plasmodesmata in the Brown Alga Dictyota dichotoma (Dictyotales, Phaeophyceae). Planta 236: 1013-1026.
3.Fu, G., Nagasato, C., Ito, T., M?ller, D. G., Motomura, T. (2012) Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae). Protoplasma DOI 10.1007/s00709-012-0405-7.


1.Katsaros, C., Varvarigosa, V., Gachon, C. M. M., Jerry Brand, Motomura, T., Nagasato, C., and K?pper, F. C. (2011) Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus (Chlorophyta).
Protist. 162, 315-331.


1.Kimura, K., Nagasato, C., Shinya, U. and Motomura, T. (2010) Sperm mitochondrial DNA elimination in the zygote of the oogamous brown alga Undaria pinnatifida (Laminariale, Phaeophyceae). Cytologia. 75: 353-361.
2.Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K., and Motomura, T. (2010) Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). Planta 232: 287-298.
3.Terauchi, M., Kato, A., Nagasato, C. and Motomura, T. (2010) Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta). Phycological Research 58: 217-221.
4.Cock, J. M., Sterck, L., Rouz?, P., Scornet, D., Allen, A. E., Amoutzias, G., Anthouard, V., Artiguenave, F., Aury, J.-M., Badger, J. H., Beszteri, B., Billiau, K., Bonnet, E., Bothwell, J. H. F., Bowler, C., Boyen, C., Brownlee, C., Carrano, C. J., Charrier, B., Cho, G. Y., Coelho, S. M., Coll?n, J., Corre, E., Da Silva, C., Delage, L., Delaroque, N., Dittami, S. M., Doulbeau, S., Elias, M., Farnham, G., Gachon, C. M.M., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., K?pper, F. C., Lang, D., Le Bail, A., Leblanc, C., Lerouge, P., Lohr, M., Lopez, P. J., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C. A., Nelson, D. R., Nyvall-Coll?n, P., Peters, A. F., Pommier, C., Potin, P., Poulain, J., Quesneville, H., Read, B., Rensing, S. A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D. C., S?gurens, B., Strittmatter, M., Tonon, T., Tregear ,J., Valentin, K., von Dassow, P., Yamagishi, T., de Peer, Y. V. and Wincker, P. (2010) The Ectocarpus genome and the independent evolution of multicellularity in the brown algae. Nature 465:617-621.
5.Motomura, T., Nagasato, C. and Kimura, K. (2010) Cytoplasmic inheritance of organelles in brown algae. Journal of Plant Research 123:185-192.
6.Kimura, K., Nagasato, C., Kogame, K. and Motomura, T. (2010) Disappearance of male gamete mitochondrial DNA after four-celled stage sporophyte of the isogamous brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Journal of Phycology 46:143-152.
7.Kato, H, Motomura, T., Komeda, Y., Saito, T. and Kato, A. (2010) Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana.
Journal of Plant Physiology 167: 571?577.
8.植木知佳,村上明男,加藤敏朗,嵯峨直恆,本村泰三 (2010) 紅藻スサビノリの光合成色素と葉緑体微細構造における栄養欠乏応答.日本水産学会誌76:375-382.


1.Yamagishi, T., Motomura, T., Nagasato, C. and Motomura, T. (2009) Novel proteins composing the stramenopile tripartite mastigoneme in Ochromonas danica (Chrysophyceae). Journal of Phycology 45:1100-1105.
2.Katsaros, C., Motomura, T., Nagasato, C. and Galatis, B. (2009) Diaphragm development in cytokinetic vegetative cells of brown algae. Botanica Marina 52:150-161.
3.Motomura, T. and Nagasato, C. (2009) Functional and non-functional spindle formation in Fucus distichus zygotes: the role of centrosome. Botanica Marina 52:140-149.
4.Ishikawa, M., Takahashi, F., Nozaki, H., Nagasato, C., Motomura, T. and Kataoka, H. (2009) Distribution and phylogeny of the blue light receptors aureochromes in eukaryotes. Planta 230: 543-552.
5.Nagasato, C. and Motomura, T. (2009) Effect of latrunculin B and brefeldin A on cytokinesis in the brown alga, Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Journal of Phycology 45:404-412.
6.Ueki, C., Nagasato, C., Motomura, T. AND Saga, N. (2009) Ultrastructure of mitosis and cytokinesis during spermatogenesis in Porphyra yezoensis (Bangiales, Rhodophyta). Botanica Marina 52:129-139.
7.北山茂一、原宏哉、岩城美朝、長里千香子、本村泰三、遠山忠、Chang Young-Choel、菊池慎太郎(2009)石油系燃料油汚染土壌のバイオレメディエーション ―特に環境微生物学的なアプローチ― 環境技術(Journal of Environmental conservation Engineering)38:52-58.
8.Yamamoto, Y. Y., Yusa, Y., Yamamoto. S., Hirano, Y., Hirano, Y., Motomura, T., Tanemura, T. and Obokata, J. (2009) Identification of photosynthetic sacoglossans from Japan. Encocytobiosis Cell Res. 19:112-119.