研究内容

私たちの研究室ではコンブ、ワカメなどが含まれる褐藻類の細胞生物学的、分子生物学的研究を行っています。褐藻類は分類学的にはストラメルパイル系統群不等毛藻類門褐藻網に属し、複雑な世代交代と生殖様式を持った多細胞体制の海藻です。沿岸生態系において炭素固定を行う主要な一次生産者であるとともに、重金属の吸着、窒素・リンの吸収を行うことからバイオレメディエーションへの応用にも注目されている生物群です。褐藻類は地球水圏において最も大型化を成し得た多細胞光合成生物であり、その体制を機械的・機能的に支える粘性のある細胞壁構成多糖類は、褐藻類の生物学的特徴の一つとしてあげられます。多細胞体制をとる生物は起源をたどると一つの生殖細胞に由来し、細胞分裂によって生じた娘細胞が分化と協調性をもって固有の体制を構築しています。私たちの研究室では褐藻類における多細胞体制の構築と維持に関して理解するため、①細胞質分裂面形成システムの研究、②原形質連絡の構造と機能に関する研究、③細胞壁タンパク質のプロテオミクスを進めています。さらに、褐藻類の持続的な資源確保には、生活環の完結が不可欠であることから、④世代間の橋渡しを担う生殖細胞の形成・発生、⑤雌雄配偶子の認識機構の解明、⑥栄養生理実験による効率的な成熟、成長条件の探索を行っています。

細胞質分裂面形成システムの研究

褐藻類の細胞質分裂の進行過程は長い間、明らかにされていませんでした。中心体を有し、細胞質分裂面にアクチンが出現する褐藻類では、細胞質分裂は動物、菌類と同様に収縮環による細胞質分裂が行われていると理解されてきました。しかしながら当研究室で行った研究から、褐藻類の細胞質分裂は、ゴルジ体由来小胞と平板小嚢（褐藻類の細胞質分裂に関与する扁平な膜構造）の融合により分裂面の内側から外側に向けて進行していくことが明らかになりました。本研究テーマでは隔膜形成過程、セルロースおよび褐藻類に特異的な細胞壁多糖類であるアルギン酸、フコイダンの蓄積過程について調べ、褐藻類の細胞質分裂時に出現する平板小嚢の起源と機能について明らかにしようとしています。

原形質連絡の構造と機能に関する研究

動物のギャップ結合や緑色植物の原形質連絡、紅藻のピットプラグのように、多細胞生物には細胞間連絡構造が存在しています。褐藻類にも隣接する細胞同士で細胞膜が細胞壁内を貫くように連結した原形質連絡が存在していますが、陸上植物のものとは異なり、小胞体から派生するデスモ小管は見られません。褐藻類における原形質連絡は、陸上植物と同様に厚い細胞壁で覆われた細胞間の物質移動に関わっていると考えられていますが、詳細については未だ明らかにされていません。本研究テーマでは原形質連絡の構造と機能について、その形成過程と出現パターンの関係から明らかにしようとしています。

細胞壁タンパク質のプロテオミクス

準備中

世代間の橋渡しを担う生殖細胞の形成・発生

藻類の生活環は動物、種子植物とは大きく異なり、単相の配偶体世代と複相の胞子体世代が独立しています。二つの世代を結んでいるのが、雌雄配偶子による受精と胞子体に減数分裂を伴って形成される遊走子（四分胞子）です。二つの世代を回るサイクルにおいて連続するのはゲノムだけではなく、細胞小器官、すなわちミトコンドリア、葉緑体、セントリオールも同様です。しかし、有性生殖ではこれらオルガネラは細胞質遺伝の機構が働きます。褐藻の有性生殖は同型・異型配偶子接合、卵生殖が存在しており、それぞれの場合で細胞質遺伝にはルールがあります。まず、ミトコンドリアはすべての生殖様式で母性遺伝、セントリオールは父性遺伝となります。葉緑体では同型配偶子接合では両性遺伝です。オルガネラの分配は減数分裂を伴う遊走子においても厳密なルールがあり、減数分裂後に核と葉緑体はセントリオールを介してそれぞれが近傍に位置するようになり、同調的に分裂します。

雌雄配偶子の認識機構の解明

生物進化の過程でいつ、どのように有性生殖が誕生したかは不明ですが、それが生物進化において必須であったことは確かです。多くの生物で、雄性配偶子（精子）が雌性配偶子（卵）からの性フェロモンによって誘引され、お互いを認識して受精（細胞融合）が起こることはよく知られています。褐藻の有性生殖では雌性配偶子からのエクトカルペンやフコセラテンなどの低分子疎水性炭化水素によって雄性配偶子が誘因されることが、ドイツ人藻類学者のミューラー博士等により報告されてきました。しかし、雄性配偶子に存在すると考えられる受容体についてはまったく未知のままです。褐藻の配偶子は不等毛植物の名の通り、マスチゴネマの付着した長い前鞭毛と短い後鞭毛を持っています。受精の時には、雄性配偶子の前鞭毛が雌性配偶子表面をなでるような運動を行うことも観察されてきました。現在、フェロモン受容体は雄性配偶子の鞭毛に存在しているのではないかという仮説のもとに、雌雄配偶子から鞭毛を単離し、LC-MS/MSによる比較プロテオミクスにより研究を進めています。

栄養生理実験による効率的な成熟、成長条件の探索

海藻（緑藻、紅藻、褐藻）は陸上植物と大きく異なり、それぞれが特徴的な生活環を有しています。例えば、コンブやワカメは巨視的胞子体と微視的雌雄配偶体が独立して存在しています。つまり、フィールドでの藻場の造成・維持管理を考える時には、独自の生活環とその制御を考えた上での対策が必要となります。海藻の場合、種を蒔けば生えてくる訳ではありません。北海道日本海側の沿岸域では海の砂漠化と呼ばれている磯焼けが進行しています。室蘭臨海実験所は、海藻類の無菌培養、栄養生理、生活環制御について長く研究を進めてきた研究室です。そこで、海水中の鉄をキーワードとした藻場再生事業を新日本製鐵住金とともに共同研究を展開しています。海洋において鉄は藻類の生長にとって制限要因であることは良く知られています。コンブを例にとると、１）胞子体から放出された遊走子は鉄に対して正の走化性を持つこと、２）雌雄配偶体は培地中の鉄により成熟が誘導されること、３）胞子体の成長に鉄は必須であることが明らかとなっています。これら厳密にコントロールされた室内実験での結果をフィールドに反映させた形で実際のフィールドでの藻場再生にチャレンジしています。

研究業績

2024

2.	Moromizato, R., Fukuda, K., Suzuki, S., Motomura, T., Nagasato, C., & Hirakawa, Y. Pyrenoid proteomics reveals independent evolution of the CO2-concentrating organelle in chlorarachniophytes. Proceedings of the National Academy of Sciences, 121, e2318542121. https://doi.org/10.1073/pnas.2318542121
1.	Takeuchi, Y., Sato, S., Nagasato, C., Motomura, T., Okuda, S., Kasahara, M., Fumio Takahashi & Yoshikawa, S. Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri. Scientific Reports, 14, 3387. https://doi.org/10.1038/s41598-024-53729-2

2023

5.	Horinouchi, Y., & Togashi, T. Unicellular and multicellular developmental variations in algal zygotes produce sporophytes. Biology Letters, 19, 20230313. https://doi.org/10.1098/rsbl.2023.0313
4.	Togashi, T., Parker, G. A., & Horinouchi, Y. Mitochondrial uniparental inheritance achieved after fertilization challenges the nuclear–cytoplasmic conflict hypothesis for anisogamy evolution. Biology Letters, 19, 20230352. https://doi.org/10.1098/rsbl.2023.0352
3.	Shen, Y., Motomura, T., Ichihara, K., Matsuda, Y., Yoshimura, K., Kosugi, C., & Nagasato, C. Application of CRISPR-Cas9 genome editing by microinjection of gametophytes of Saccharina japonica (Laminariales, Phaeophyceae). Journal of Applied Phycology. https://doi.org/10.1007/s10811-023-02940-1
2.	Godfroy, O., Zheng, M., Yao, H., Henschen, A., Peters, A. F., Scornet, D., Colin, S., Ronchi, P., Hipp, K., Nagasato, C., Motomura T., Cock, M. & Coelho, S. M. The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus. Development, 150. https://doi.org/10.1242/dev.201283
1.	Aoki, H., Katsaros, C., Motomura, T., & Nagasato, C. Simultaneous visualization of the actin plate and new cell partition membrane during cytokinesis in the brown alga Sphacelaria rigidula (Sphacelariales, Phaeophyceae). Phycological Research. https://doi.org/10.1111/pre.12512

2022

6.	Nagasato, C., Yonamine, R., & Motomura, T. Ultrastructural observation of cytokinesis and plasmodesmata formation in brown algae. Plant Cell Division: Methods and Protocols, 253-264.
5.	Innami, R., Miyamura, S., Okoshi, M., Nagumo, T., Ichihara, K., Yamazaki, T., & Kawano, S. Gamete dimorphism of the isogamous green alga (Chlamydomonas reinhardtii), is regulated by the mating type-determining gene, MID. Communications Biology, 5, 1333. https://doi.org/10.1038/s42003-022-04275-y
4.	Horinouchi, Y., & Togashi, T. Invisible grazers of seaweed propagules. Ecology, 103, e3732. https://doi.org/10.1002/ecy.3732
3.	Mazeas, L., Yonamine, R., Barbeyron, T., Henrissat, B., Drula, E., Terrapon, N., Nagasato, C., & Herve, C. (2022, March). Assembly and synthesis of the extracellular matrix in brown algae. In Seminars in Cell & Developmental Biology. Academic Press.
2.	Shen, Y., Motomura, T., & Nagasato, C. Ultrastructural observations of mitochondrial morphology through the life cycle of the brown alga, Mutimo cylindricus (Cutleriaceae, Tilopteridales) Protoplasma 259, 371-383. https://doi.org/10.1007/s00709-021-01679-1
1.	Kim, G. H., Nagasato, C., Kwak, M., Lee, J. W., Hong, C. Y., Klochkova, T. A., & Motomura, T. Intercellular transport across pit-connections in the filamentous red alga Griffithsia monilis. Algae 37, 75-84. https://doi.org/10.4490/algae.2022.37.2.16

2021

5.	Ichihara,K., Yamazaki, T. & Kawano, S. Genome editing using a DNA-free clustered regularly interspaced short palindromic repeats-Cas9 system in green seaweed Ulva prolifera. Phycological Research 70, 50-56. 10.1111/pre.12472
4.	Badis, Y., Scornet, D., Harada, M., Caillard, C., Godfroy, O., Raphalen, M., Gascon, C., Coelho, S., Motomura, T., Nagasato, C., & Cock, J. M. Targeted CRISPR-Cas9-based gene knockouts in the model brown alga Ectocarpus. New Phytologist 231, 2077-20913. 10.1111/nph.17525
3.	Yonamine, R., Ichihara, K., Tsuyuzaki, S., Herve, C., Motomura, T., & Nagasato, C. Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes. Journal of Phycology 57, 1356-1367. https://doi.org/10.1093/molbev/msz186
2.	Zhang, J., Yotsukura, N., Jueterbock, A. Hu, Z., Assis, J., Nagasato, C., Yao, J. & Duan, D. Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina japonica. J Appl Phycol 33, 693-702 . https://doi.org/10.1007/s10811-020-02300-3
1.	Shen, Y., Iwao, T., Motomura, T. & Nagasato, C. Cytoplasmic inheritance of mitochondria and chloroplasts in the anisogamous brown alga Mutimo cylindricus (Phaeophyceae). Protoplasma 258, 19-32. https://doi.org/10.1007/s00709-020-01540-x

2020

2.	Choi, J.W., Graf, L., Peters, A.F., Cock, J.M., Nishitsuji, K., Arimoto, A.,Shoguchi, E., Nagasato, C., Choi, C.G. & Yoon, H.S. Organelle inheritance and genome architecture variation in isogamous brown algae. Sci Rep 10, 2048. https://doi.org/10.1038/s41598-020-58817-7
1.	Nagasato, C., Kawamoto, H., Tomioka, T., Tsuyuzaki, S., Kosugi, C., Kato., T & Motomura, T. Quantification of laminarialean zoospores in seawater byreal-time PCR. Phycological Research 68:57?62. https://doi.org/10.1111/pre.12384

2019

7.	Miyamura, S., Ichihara, K., Yamazaki, T., Kuwano, K. & Kawano, S. Visualization of Gamete Mating Structure of Marine Green Alga by FE-SEM. Cytologia 9: 13523. https://doi.org/10.1038/s41598-019-50070-x
6.	Mignerot, L., Nagasato, C., Peters, A., Perrineau, M., M., Scornet, D., Pontheaux, F., Djema, W., Roze, D., Motomura. T., Coelho, S., M., Cock, J., M. Unusual patterns of mitochondrial inheritance in the brown alga Ectocarpus. Molecular Biology and Evolution 36: 2778?2789. https://doi.org/10.1093/molbev/msz186
5.	Ichihara, K., Yamazaki, T., Miyamura, S., Hiraoka, M. & Kawano, S. Asexual thalli originated from sporophytic thalli via apomeiosis in the green seaweed Ulva. Scientific Report 9: 13523. https://doi.org/10.1038/s41598-019-50070-x
4.	Zhang, J., Yao, J., Hu, Z.‐M., Jueterbock, A., Yotsukura, N., Krupnova, T., N., Nagasato., C. & Duan, D. Phylogeographic diversification and postglacial range dynamics shed light on the conservation of the kelp Saccharina japonica. Evolutionary Applications. 12:791?803. https://doi.org/10.1111/eva.12756
3.	Sahul, H., S., Wakayama, M., Ichihara, K., Sakurai, K., Ashino, Y., Kadowaki, R., Soga, T. and Tomita, M. Metabolome profling of various seaweed species discriminates between brown, red, and green algae. Planta 249: 1921-1947
2.	Kinoshita-Terauchi, N., Shiba, K., Umezawa, T., Matsuda, F., Motomura, T. and Inaba, K. A brown algal sex pheromone reverses the sign of phototaxis by cAMP/Ca2+-dependent signaling in the male gametes of Mutimo cylindricus (Cutleriaceae). Journal of Photochemistry and Photobiology B: Biology 192: 113-123
1.	Klochkova, T., A., Motomura, T., Nagasato, C., Klimova, A., V. and Kim, G., H. The role of egg flagella in the settlement and development of zygotes in two Saccharina species. Phycologia. Published online. https://doi.org/10.1080/00318884.2018.1528804

2018

4.	Zhang, J., Yao, J. Hu, Zi-Min, Jueterbock, A., Yotsukura, N., Krupnova, T. Nagasato, C. and Duan, De-Lin. (2018) Phylogeographic diversification and postglacial range dynamics shed light on the conservation of the kelp Saccharina japonica . Evolutionary Applications .12:791?803
3.	Cui, J., Monotilla, A., P., Zhu, W., Takano, Y., Shimada, S., Ichihara, K., Matsui, T., He, P., and Hiraoka, M. (2018) Taxonomic reassessment of Ulva prolifera (Ulvophyceae, Chlorophyta) based on specimens from the type locality and Yellow Sea green tides. Phycologia 57: 692-704.
2.	Nagasato, C. and Motomura, T. (2018) Effect of brefeldin A and the dynamics of the actin plate on cytokinesis of zygotes in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). European Journal of Phycology. doi.org/10.1080/09670262.2018.1501516
1.	Nagasato, C., Katsaros, C. and Motomura, T. (2018) Cryofixation of brown algae for transmission electron microscopy. In Protocols for macroalgae research [eds Charrier, B., Wichard, T. and Reddy, C. R. K.], CRC Press. Taylor & Francis Group.

2017

9.	Godfroy, O., Uji, T., Nagasato, C., Lipinska, A. P., Scornet, D., Peters, A. F., Avia, K., Colin, S., and Mignerot L., Motomura, T., Cock, J. M. and Coelho, S. (2017) DISTAG/TBCCd1 is required for basal cell fate determination in Ectocarpus. Plant Cell. DOI 10.1105/tpc. 17.00440.
8.	Yamazaki, T., Ichihara, K., Suzuki, R., Oshima, K., Miyamura, S., Kuwano, K., Toyoda, A., Suzuki, Y., Sugano, S., Hattori, M. & Kawano, S. (2017) Genomic structure and evolution of the mating-type locus in the green seaweed Ulva partita. Scientific Reports 71(1): 11679. doi: 10.1038/s41598-017-11677-0.
7.	Hiraoka, M., Ichihara, K., Zhu, W., Shimada, S., Oka, N., Cui, J., Tsubaki, S. & He, P. (2017) Examination of species delimitation of ambiguous DNA-based Ulva (Ulvophyceae, Chlorophyta) clades by culturing and hybridization. Phycologia 56 (5): 517-532. doi.org/10.2216/16-109.1.
6.	Masakiyo, Y., Ogura, A., Ichihara, K., Yura, K. & Shimada S. (2017) RNA-seq analysis revealed molecular mechanism for low-salinity adaptation between closely related ulvarian seawater and brackish water distributed species. Algal Resourses 9: 61-76.
5.	Saber, A., A., Ichihara, K. & Cantonati, M. (2017) Molecular phylogeny and detailed morphological analysis of two freshwater Rhizoclonium strains from contrasting spring types in Egypt and Italy. Plant biosystems 151: 800-812. doi.org/10.1080/11263504.2016.1211195.
4.	Kinoshita, N., Nagasato, C. and Motomura, T. (2017) Phototaxis and chemotaxis of brown algal swarmers. Journal of Plant Research DOI 10.1007/s10265-017-0914-8.
3.	Kinoshita, N., Nagasato, C. and Motomura, T. (2017) Calcium control of the sign of phototaxis in brown algal gametes of Mutimo cylindricus. Photochemistry and Photobiology (in press).
2.	Iwata, I., Kimura, K., Tomaru, Y., Motomura. T., Koike, K., Koike, K. and Honda, D. (2017) Bothrosome formation in Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles) during zoospore settlement. Protist 168: 206-219.
1.	Tanaka, A., Hoshino, Y., Nagasato, C. and Motomrua, T. (2017) Branch regeneration induced by sever damage in the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae). Protoplasma 254:1341-1351.

2016

9.	Nagasato, C, Tanaka, A., Ito, T., Katsaros, C. and Motomura T. (2016) Intercellular translocation of molecules via plasmodesmata in the multiseriate filamentousbrown alga, Halopteris congesta (Sphacelariales,Phaeophyceae). Journal of Phycology DOI 10.1111/jpy.12498.
8.	長里千香子・寺内真・本村泰三（2016）多細胞藻類における一次細胞間連絡の形成．「顕微鏡」第51巻3号．
7.	Kikutani, S., Nakajima, K., Nagasato, C., Tsuji, Y., Miyatake, A. and Matsuda, M. (2016) A novel luminal θ-carbonic anhydrase critical for growth and photosynthesis in the marine diatom Phaeodactylum tricornutum. Proceedings of the National Academy of Sciences of the United States of America DOI 10.1073/pnas.1603112113.
6.	Kinoshita, N., Fu, G., Ito, T. and Motomura, T. (2016) Three-dimensional organization of flagellar basal apparatus in Ectocarpus gametes. Phycological Research 64:19-25.
5.	Kinoshita, N., Nagasato, C., Tanaka, A. and Motomura, T. (2016) Chemotaxis in the anisogamous brown alga Mutimo cylindricus. Phycologia 55:359?364.
4.	Yamada, K., Yoshikawa, S., Ohoki, K., Ichinomiya, M., Kuwata, A., Motomura, T., Nagasato, C. (2016) Ultrastructural analysis of siliceous cell wall regeneration in Triparma laevis (Parmales, Bolidophyceae). Phycologia. 55: 602-609
3.	Yamada, K., Nagasato, C., Motomura, T., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K. and Yoshikawa, S. (2016) Mitotic spindle formation in Triparma laevis NIES-2565 (Parmales, Heterokontophyta). Protoplasma. DOI 10.1007/s00709-016-0967-x
2.	Kinoshita, N., Nagasato, C. and Motomura, T. (2016). Chemotactic movement in sperm of the oogamous brown algae, Saccharina japonica and Fucus distichus. Protoplasma. DOI 10.1007/s00709-016-0974-y.
1.	Terauchi, M., Nagasato, C., Inoue, A., Ito, T and Motomura, T. (2016). Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae). Planta DOI 10.1007/s00425-016-2516-4.

2015

13.	Tanaka A, De Martino A, Amato A, Montsant A, Mathieu B, Rostaing P, Tirichine L, Bowler C. (2015) Ultrastructure and Membrane Traffic During Cell Division in the Marine Pennate Diatom Phaeodactylum tricornutum. Protist 166:506-521.
12.	Zhang, J., Yao, J. T., Sun, Z. M., Fu, G., Galanin, D. A., Nagasato, C., Motomura, T., Hu, Z. M. and Duan, D. L. (2015) Phylogeographic data revealed shallow genetic structure in the kelp Saccharina japonica (Laminariales, Phaeophyta). BMC Evol. Biol. DOI. 10.1186/s12862-015-0517-8.
11.	Iwai, H., Fukushima, M., Yamamoto, M. and Taizo Motomura, T. (2015) Seawater extractable organic matter (SWEOM) derived from a compost sample and its effect on the serving bioavailable Fe to the brown alga, Saccahrina japonica. Humic Substances Research. Humic Sub. Res. (In press)
10.	Fu G, Nagasato C, Yamagishi T, Kawai H, Okuda K, Takao Y, Horiguchi T and Motomura T. (2015). Ubiquitous distribution of helmchrome in phototactic swarmers of the Stramenopiles. Protoplasma (In press)
9.	Kinoshita N, Shiba K, Inaba K, Fu G, Nagasato C and Motomura T. (2015). Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus. Eur. J. Phycol. DOI 10.1080/09670262.2015.1109144.
8.	Bailleul B, Berne N, Murik O, Petroutsos D, Prihoda J, Tanaka A, Villanova V, Bligny R, Flori S, Falconet D, Krieger-Liskay A, Santabarbara S, Rappaport F, Joliot P, Tirichine L, Falkowski P, Cardol P, Bowler C, Finazzi G. (2015). Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms. Nature. 524:366-369
7.	Klochkova T A, Shin Y J, Moon K H, Motomura T and Kim G H. (2015). New species of unicellular obligate parasite, Olpidiopsis pyropiae sp. nov., that plagues Pyropia sea farms in Korea. J. Appl. Phycol. DOI 10.1007/s10811-015-0595-4.
6.	Nagasato C, Terauchi M, Tanaka A and Motomura T. (2015). Development and function of plasmodesmata in Fucus zygotes. Botanica Marina. 58: 229?238.
5.	Villar E, Farrant G, Follows M, Garczarek L, Speich S, Audic S, Bittner L, Blanke B, Brum JR, Brune C, Casotti R, Chase A, Dolan JR, d'Ortenzio F, Gattuso JP, Grima N, Guidi L, Hill CN, Jahn O, Jamet JL, Le Goff H, Lepoivre C, Malviya S, Pelletier E, Romagnan JB, Roux S, Santini S, Scalco E, Schwenck SM, Tanaka A, Testor P, Vannier T, Vincent F, Zingone A, Dimier C, Picheral M, Searson S, Kandels-Lewis S, Tara Oceans coordinators, Acinas SG, Bork P, Boss E, de Vargas C, Gorsky G, Ogata H, Pesant S, Sullivan MB, Sunagawa S, Wincker P, Karsenti E, Bowler C, Not F, Hingamp P and Iudicone D. (2015). Environmental characteristics of Agulhas rings affect inter-ocean plankton transport. Science. 348: 6237
4.	Huysman MJJ, Tanaka A, Bowler C, Vyverman W and De Veylder L. (2015). Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage. BMC Plant Biol. in press.
3.	Terauchi, M, Nagasato, C, and Motomura, T. (2015). Plasmodesmata of brown algae. J. Plant Res. 128:7-15.
2.	Idei, M, Sato, S, Nagasato, C, Motomura, T, Toyoda, K, Nagumo, T, amnd Mann, D, G. (2015). Spermatogenesis and auxospore structure in the multipolar centric diatom Hydrosera. J. Phycol. 51, 144-158.
1.	Iwai, H., Fukushima, M., Motomura, T., Kato, T., Kosugi, C. (2014) Effect of iron complexes with seawater extractable organic matter on oogenesis in gametophytes of brown macroalgae (Saccharina japonica). J. Appl. Phycol. 27:1583?1591.

2014

6.	J. Morrissey, R. Sutak, J. Paz-Yepes, A. Tanaka, A. Moustafa, A.j Veluchamy, Y. Thomas, H Botebol, F.Y. Bouget, J.B. McQuaid, L. Tirichine, A.E. Allen, E.l Lesuisse, and C.Bowler (2014) A Novel Protein, Ubiquitous in Marine Phytoplankton, Concentrates Iron at the Cell Surface and Facilitates Uptake. Curr. Biol. 25: 364-371.
5.	Jong Won Han, J. W., Tatyana A. Klochkova, T. A, Shim, J., Nagasato, C. Motomura, T., and Kim, G. H. (2014) Identification of three proteins involved in fertilization and parthenogenetic development of a brown alga, Scytosiphon lomentaria. Planta. DOI 10.1007/s00425-014-2148-5.
4.	Fu, G., Nagasato, C., Oka, S., Cock, M. J. and Motomura, T. (2014) Proteomics analysis of heterogeneous flagella in brown alga (Stramenopiles). Protist. DOI:10.1016/j.protis.2014.07.007.
3.	Nagasato, C., Kajimura, N., Terauchi, M., Mineyuki, Y. and Motomura, T. (2014) Electron tomographic analysis of cytokinesis in the brown alga Silvetia babingtonii (Fucales, Phaeophyceae). Protoplasma DOI 10.1007/s00709-014-0635-y.
2.	Sato, T., Nagasato, C., Hara, Y. and Motomura, T. (2014) Cell cycle and nucleomorph division in Pyrenomonas helgolandii (Cryptophyta).Protist 165:113-122.
1.	Fu, G., Kinoshita, N., Nagasato, C. and Motomura, T. (2014) Fertilization of brown algae: Flagellar function in phototaxis and chemotaxis. In Sexual Reproduction in Animals and Plants. (eds. Sawada, H. Inoue, N., and Iwano, M.) Springer Open.

2013

2.	Klochkova, T., Kwak, M-S, Han, J-Won, Motomura, T., Nagasato, C. and Kim, G-H. (2013) Cold-tolerant strain of Haematococcus pluvialis (Haematococcaceae, Chlorophyta) from Blomstrandhalv?ya (Svalbard). Algae 28, 185-192.
1.	Fu, G., Nagasato, C., Ito, T., Muller, D. G. and Motomura, T. (2013) Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae). Protoplasma 250:261-272.

2012

2.	Kitauchi, T., Saito, T., Motomura, T., Arai, K., Yamaha, E. (2012) Distribution and function of germ plasm in cytoplasmic fragments from centrifuged eggs of the goldfish, Carassius auratus. J. Appl. Ichthyol.28:998-1005.
1.	Terauchi, M., Nagasato, C., Kajimura, N., Mineyuki, Y., Okuda, K., Katsaros, C., Motomura. T. (2012) Ultrastructural Study of Plasmodesmata in the Brown Alga Dictyota dichotoma (Dictyotales, Phaeophyceae). Planta 236: 1013-1026.

2011

1.	Katsaros, C., Varvarigosa, V., Gachon, C. M. M., Jerry Brand, Motomura, T., Nagasato, C., and K?pper, F. C. (2011) Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus (Chlorophyta). Protist 162, 315-331.

2010

8.	Kimura, K., Nagasato, C., Shinya, U. and Motomura, T. (2010) Sperm mitochondrial DNA elimination in the zygote of the oogamous brown alga Undaria pinnatifida (Laminariale, Phaeophyceae). Cytologia 75: 353-361.
7.	Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K., and Motomura, T. (2010) Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). Planta 232: 287-298.
6.	Terauchi, M., Kato, A., Nagasato, C. and Motomura, T. (2010) Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta). Phycological Research 58: 217-221.
5.	Cock, J. M., Sterck, L., Rouz?, P., Scornet, D., Allen, A. E., Amoutzias, G., Anthouard, V., Artiguenave, F., Aury, J.-M., Badger, J. H., Beszteri, B., Billiau, K., Bonnet, E., Bothwell, J. H. F., Bowler, C., Boyen, C., Brownlee, C., Carrano, C. J., Charrier, B., Cho, G. Y., Coelho, S. M., Coll?n, J., Corre, E., Da Silva, C., Delage, L., Delaroque, N., Dittami, S. M., Doulbeau, S., Elias, M., Farnham, G., Gachon, C. M.M., Gschloessl, B., Heesch, S., Jabbari, K., Jubin, C., Kawai, H., Kimura, K., Kloareg, B., K?pper, F. C., Lang, D., Le Bail, A., Leblanc, C., Lerouge, P., Lohr, M., Lopez, P. J., Martens, C., Maumus, F., Michel, G., Miranda-Saavedra, D., Morales, J., Moreau, H., Motomura, T., Nagasato, C., Napoli, C. A., Nelson, D. R., Nyvall-Coll?n, P., Peters, A. F., Pommier, C., Potin, P., Poulain, J., Quesneville, H., Read, B., Rensing, S. A., Ritter, A., Rousvoal, S., Samanta, M., Samson, G., Schroeder, D. C., S?gurens, B., Strittmatter, M., Tonon, T., Tregear ,J., Valentin, K., von Dassow, P., Yamagishi, T., de Peer, Y. V. and Wincker, P. (2010) The Ectocarpus genome and the independent evolution of multicellularity in the brown algae. Nature 465:617-621.
4.	Motomura, T., Nagasato, C. and Kimura, K. (2010) Cytoplasmic inheritance of organelles in brown algae. Journal of Plant Research 123:185-192.
3.	Kimura, K., Nagasato, C., Kogame, K. and Motomura, T. (2010) Disappearance of male gamete mitochondrial DNA after four-celled stage sporophyte of the isogamous brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Journal of Phycology 46:143-152.
2.	Kato, H, Motomura, T., Komeda, Y., Saito, T. and Kato, A. (2010) Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana. Journal of Plant Physiology 167: 571?577.
1.	植木知佳，村上明男，加藤敏朗，嵯峨直恆，本村泰三 (2010) 紅藻スサビノリの光合成色素と葉緑体微細構造における栄養欠乏応答．日本水産学会誌76：375-382.

2009

8.	Yamagishi, T., Motomura, T., Nagasato, C. and Motomura, T. (2009) Novel proteins composing the stramenopile tripartite mastigoneme in Ochromonas danica (Chrysophyceae). Journal of Phycology 45:1100-1105.
7.	Katsaros, C., Motomura, T., Nagasato, C. and Galatis, B. (2009) Diaphragm development in cytokinetic vegetative cells of brown algae. Botanica Marina 52:150-161.
6.	Motomura, T. and Nagasato, C. (2009) Functional and non-functional spindle formation in Fucus distichus zygotes: the role of centrosome. Botanica Marina 52:140-149.
5.	Ishikawa, M., Takahashi, F., Nozaki, H., Nagasato, C., Motomura, T. and Kataoka, H. (2009) Distribution and phylogeny of the blue light receptors aureochromes in eukaryotes. Planta 230: 543-552.
4.	Nagasato, C. and Motomura, T. (2009) Effect of latrunculin B and brefeldin A on cytokinesis in the brown alga, Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Journal of Phycology 45:404-412.
3.	Ueki, C., Nagasato, C., Motomura, T. AND Saga, N. (2009) Ultrastructure of mitosis and cytokinesis during spermatogenesis in Porphyra yezoensis (Bangiales, Rhodophyta). Botanica Marina 52:129-139.
2.	北山茂一、原宏哉、岩城美朝、長里千香子、本村泰三、遠山忠、Chang Young-Choel、菊池慎太郎（2009）石油系燃料油汚染土壌のバイオレメディエーション　―特に環境微生物学的なアプローチ―　環境技術（Journal of Environmental conservation Engineering）38:52-58.
1.	Yamamoto, Y. Y., Yusa, Y., Yamamoto. S., Hirano, Y., Hirano, Y., Motomura, T., Tanemura, T. and Obokata, J. (2009) Identification of photosynthetic sacoglossans from Japan. Encocytobiosis Cell Res. 19:112-119.

2008

2.	Ueki, C., Nagasato, C., Motomura, T. and Saga, N. (2008) Reexamination of the Pit Plugs and the Characteristic Membranous Structures in Porphyra Yezoensis (Bangiales, Rhodophyta). Phycologia 47:5-11.
1.	山本義治、種村尚典、遊佐陽一、平野弥生、平野義明、本村泰三、小保方潤一（2008）光合成をするウミウシ．うみうし通信　60:10-11.

2007

2.	Tanaka, A., Nagasato, C., Uwai, S., Motomura, T., and Kawai, H. (2007) Re-examination of ultrastructures of the stellate chloroplast organization in brown algae: structure and development of pyrenoids. Phycological Research 55:203-213.
1.	Yamagishi T, Nagasato, C., Motomura T, Kawai H (2007) A tubular mastigoneme-related protein, ocm1, isolated from the flagellum of a chromophyte alga, Ochromonas danica. Journal of Phycology 43: 519-527.

2006

2.	Uemori, C., Nagasato, C., Kato, A. and Motomura, T. (2006) Ultrastructural and immunocytological studies on the rhizoplast in the chrysophycean alga Ochromonas danica. Phycological Research 54 (2): 133-139.
1.	Kato, Y., Kogame, K., Nagasato, C., and Motomura, T. (2006) Inheritance of mitochondrial and chloroplast genomes in the isogamous brown alga Scytosiphon lomentaria. Phycological Research 54 (1): 65-71.

2005

4.	Kawai, H., Motomura, T. and Okuda, K. (2005) Isolation and purification techniques for macroalgae. In Andersen, R. A. (eds) Algal culturing techniques. Elsevier academic press, San Diego, pp 133-143.
3.	Nagasato, C. (2005) Behavior and function of paternally inherited centrioles in brown algal zygotes. Journal of　Plant Research 118:361-369.
2.	Uwai, S., Nagasato, C., Motomura, T. and Kogame, K. (2005) Life history and molecular phylogenetic relationships of Asterocladon interjectum sp. nov. (Phaeophyceae). European Journal of Phycology 40 (2):179-194.
1.	Fujita, S., Iseki, M., Yoshikawa, S., Makino, Y., Watanabe, M., Motomura, T., Kawai, H. and Murakami, A. (2005) Identification and characerization of a fluorescent flagellar protein from the brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae): A flavoprotein homologous to old yellow enzyme. European Journal of Phycology 40: 159-167.